Maria Virgen: Blog 9 : Transformation and Transduction

In bacterial transformation, bacteria and DNA are mixed together to create a "heat shock". "heat shock" is a process that induces the bacteria to take in plasmid DNA. With the ionic shield in place, the DNA is swept through the adhesion zone(admit plasmid DNA). Only transformed bacteria that contain two types of resistance genes could grow in the presence of the antibodies, tetracyline and kanamycin. As a result after bacterial transformation was a recombined plasmid containing both the (tet) and the (kan) gene. Same methods could be used recombine genes from eukaryotic and prokaryotic organisms.
Transduction occurs in the lytic cycle or the lysogenic cycle. In Transduction, genetic information is transferred between cells using bacteriophage ( virus particle). It does not require cell to cell contact and does not demote DNA. Specialized transduction uses specialized phage, in which some of the phage's genetic material has been replaced by bacterial chromosome. The phage particles carry the same amount of the bacterial chromosome(genetic material). The phage can introduce their DNA into the recipient bacterium through recombination,the breaking of genetic material to join other genetic material. Finally, host cell obtains phage DNA and genes from the previous host.